A Comparative Study between Microwave Hydrodiffusion and Gravity (MHG) and Ultrasound-Assisted Extraction (UAE): Chemical and Biological Characterization of Polyphenol-Enriched Extracts from Aglianico Grape Pomace

The wine industry produces large amounts of grape pomace (GP), a waste that needs to be disposed properly. Bioactive compounds with high added value can recovered from GP as an interesting strategy reduce the environmental impact. Here, two different technologies were employed recover polyphenol GP: microwave hydrodiffusion and gravity (MHG) ultrasound-assisted extraction (UAE). further purification UAE MHG extracts was carried out through solid-phase (SPE) obtain three fractions, F1, F2 F3. ATR-FTIR analysis confirmed presence sugar polysaccharide components in well non-anthocyanin anthocyanin F3, respectively. Also, chemical profile determined by HPLC-UV-DAD, identifying catechin F2, malvidin-3-O-glucoside chloride cyanidin derivative main fractions their parental characterized for total phenolic content (TPC) scavenger activity vitro assays. We found F2-MHG F3-MHG contained phenol contents 6.5 8.5 times higher than those non-fractionated extracts. Finally, (100 μg/mL, w/v) shown proliferation HT-29 cells.